추가 정보 영역
| Speaker |
Sungchul Hohng |
| Affiliation |
Seoul National University |
| URL |
Link |
| Date |
May 17, 2018 |
| Time |
4:00 pm - 5:00 pm |
| Venue |
BLDG 110 ROOM N104 |
| Sponsor |
UNIST-Life Sciences |
| Host |
- |
| Contact |
- |
| Phone |
- |
Chromodomain-helicase-DNA-binding protein 1 (CHD1) remodels chromatin by translocating nucleosomes along DNA, but its mechanism remains poorly understood. We employed a single-molecule fluorescence approach to characterize nucleosome remodeling by yeast CHD1 (Chd1p). We show that Chd1p translocates nucleosomes in steps of multiple base pairs per ATP. ATP binding to Chd1p induces a transient unwrapping of the exit-side DNA, and facilitatesnucleosome translocation. ATP hydrolysis induces nucleosome translocation, which is followed by the rewrapping upon the release of the hydrolyzed nucleotide. Multiple Chd1ps binding to a single nucleosome sequentially moves a histone octamer with a preference to the center of DNA fragments, suggesting a new mechanism for regularly spaced nucleosome generation by Chd1p. Our results reveal the unique mechanism by which Chd1p remodels nucleosomes. At the beginning of the talk, I will also briefly overview the research topics in my laboratory.
