Seminars & Speakers

Cell Migration and Gene Expression - studying single cells on artificial micro-pattern
Speaker Joachim O. Rädler
Affiliation Physics Faculty and Center for NanoScience, Ludwig-Maximilians-Universität München
URL Link
Date March 15, 2019
Time 2:00 pm
Venue Bldg. 103, Rm. 309
Sponsor IBS-Center for Soft and Living Matter
Host Steve Granick
Contact raedler@lmu.de
Phone 052-217-5527
Attach New_Seminar_announcement_Radler.pdf_page_1.jpg (598.9K)
Artificial micro-patterns with defined geometries are being used to build platforms to study cell migration at the single cell level. We present time-lapse microscopy studies of cell migration on short stripes, ring-shaped micro-lanes, ring-shaped micro-lanes with a built-in artificial chemical barrier as well as dumbbell pattern. The cell motion is analyzed in one dimension using a two-state model and compared to Potts Model computer simulations. Small groups of cells in mesoscale circular micro-pattern bridge from single cell migration to collective cell dynamics showing spontaneous emergence of rotational migration. Secondly we use automated time-lapse microscopy in combination with micro-patterned surfaces to trap cells for efficient high-throughput monitoring of fluorescent reporter signals at the single-cell level. We show that measurement of individual gene expression time traces yields access to mRNA translation efficiency, mRNA lifetime and delivery delay times.