Seminars & Speakers
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게시물 정보
글제목 :
Pluripotent stem cells into somatic cells; application of pluripotent stem cells
추가 정보 영역
Speaker | Jong Hee Lee |
Affiliation | KRIBB |
URL | Link |
Date | March 21, 2019 |
Time | 4:00 pm |
Venue | Bldg.110 Rm. N104 |
Sponsor | UNIST-Life Sciences |
Host | Hyung Moo Kwon |
Contact | hmkwon@unist.ac.kr |
Phone | 2535 |
Attach | 콜로퀴움 포스터.jpg (2.2M) |
We also present that the naive state enhances self-renewal while restricting lineage differentiation in vitro to neural default fate. Molecular analyses indicate expression of multiple lineage-associated transcripts in naive hPSCs that failed to predict biased functional differentiation capacity. Suppression of OCT4 but not NANOG allows immediate recovery directly from naive state. Our study reveals unique cell-fate restrictions in human pluripotent states and provides an approach to overcome these barriers that harness both efficient naive hPSC growth while maintaining in vitro differentiation essential for hPSC applications.
Induced pluripotent stem cell reprogramming has provided critical insights into disease processes by modeling the genetics and related clinical pathophysiology. Human cancer represents highly diverse genetics, as well as inter- and intra-patient heterogeneity, where cellular model systems capable of capturing this disease complexity would be invaluable. We report our efforts to induce pluripotency from the heterogeneous population of human patients that represents this disease in the clinic. Using robust optimized reprogramming methods, we demonstrate that reprogramming of AML cells harboring leukemic genomic aberrations is a rare event with the exception of those with de novo mixed-lineage leukemia (MLL) mutations that can be reprogrammed and model drug responses in vitro.